Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

BLAST program is widely used tool to find protein and DNA databases for sequence similarities. For protein comparisons, various definitions, algorithmic and statistical improvements described here allows the BLAST program execution time will decrease substantially while increasing their sensitivity to weak similarity.

New criteria to trigger the expansion of word hits, combined with new heuristics to generate gapped alignments, resulting in a gapped BLAST program that runs about three times the original speed.

Moreover, the method was introduced to automatically incorporate statistically significant alignments generated by BLAST into position-specific score matrix, and search the database using this matrix.

The resulting Position-Specific Iterated BLAST (PSI-BLAST) program running at approximately the same rate per iteration as gapped BLAST, but in many cases much more sensitive to weak but biologically relevant sequence similarities. PSI-BLAST is used to some new and interesting members of BRCT reveal superfamily.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

A method has been designed for electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method produces quantitative transfer of ribosomal protein gel containing urea. For sodium dodecyl sulfate gels, the original band pattern is obtained without loss of resolution, but the transfer is not quantitative.

This method allows the detection of proteins by autoradiography and simpler than conventional procedures. The immobilized proteins were detected by immunological procedures.

All additional binding capacity on the nitrocellulose was blocked with excess protein; then the specific antibody bound and, finally, a second antibody directed against the first antibody. The second antibody either radioactively labeled or fluorescein or peroxidase conjugate.

Certain proteins are then detected by either autoradiography, under UV light, or with a peroxidase reaction product, respectively. In the latter case, as little as 100 pg of protein clearly detectable. It is anticipated that the procedure will be applicable to the analysis of various proteins with a particular reaction or a ligand.

High-resolution two-dimensional electrophoresis of proteins.

A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Because the resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from biological sources are complex.

Proteins are separated according to the isoelectric point by isoelectric focusing in the first dimension, and according to molecular weight by sodium dodecyl sulfate electrophoresis in the second dimension. Since these two parameters are not related, it is possible to obtain nearly uniform distribution of protein spots on the gel-diminsional.

This technique has been completed in 1100 the different components of Escherichia coli and should be able to complete a maximum of 5000 proteins. A protein that contains as little as one disintegration per minute either 14C or 35S can be detected by autoradiography. A protein which is 10 minus 4 to 10 minus 5% of the total protein can be detected and quantified by autoradiography.

Reproducibility enough separation to allow any place in one of the separation to be matched to a different place in the separation. This technique provides a method to estimate (on the sensitivity described) of the amount of protein made by biological systems.

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

This system can cope with different proteins in a single charge and can consequently be used in the analysis of in vivo modification results in changes in the cost.

Proteins are responsible for altered by missense mutations can be identified. A detailed description of the methods and characteristics of this system is presented.

Comparative protein modeling by satisfaction of spatial restrictions.

We describe a comparative protein modeling methods designed to find the most likely structure for a particular sequence alignment with related structures.

Three-dimensional (3D) model is obtained with a satisfactory optimal spatial limitation comes from the harmony and expressed as a probability density function (PDF) for a feature retained. For example, the probability for the main-chain conformation residues models can be controlled by the type residue, main-chain conformation of similar residues in proteins related, and local similarity between two sequences.

Some PDF is obtained from the correlation between structural features in 17 families of homologous proteins that are aligned on the basis of their 3D structure. The PDF withstand C-C distance alpha alpha, main-chain N-O distance, the main-chain and side chain dihedral angles.

A smoothing procedure is used in the derivation of this relationship is to minimize problems rarely database. 3D models of protein molecules obtained by optimizing pdf restrictions that violate the model inputs as little as possible.

Active AHCY, human recombinant

7527-50
EUR 479

Active Recombinant Human CETP

7606-100
EUR 805

Active Recombinant Human CETP

7606-1000
EUR 4345

Active Recombinant Human CETP

7606-20
EUR 262

Active SIRT2, human recombinant

7698-10
EUR 262

Active SIRT2, human recombinant

7698-1000
EUR 4394

Active SIRT2, human recombinant

7698-50
EUR 457

Active SIRT7, human recombinant

7846-10
EUR 218

Active SIRT7, human recombinant

7846-50
EUR 588

Irisin, Active, human recombinant

7852-10
EUR 370

Irisin, Active, human recombinant

7852-50
EUR 1077

PRMT1, human recombinant (Active)

4865-10
EUR 153

PRMT1, human recombinant (Active)

4865-1000
EUR 3834

PRMT1, human recombinant (Active)

4865-50
EUR 392

GAPDH, Active, human recombinant

P1100-10
EUR 142

GAPDH, Active, human recombinant

P1100-50
EUR 392

Active Cathepsin D, Human Recombinant

9229-10
EUR 245

Active Cathepsin D, Human Recombinant

9229-100
EUR 1132

Active Cathepsin D, Human Recombinant

9229-50
EUR 735

MMP-14, Active, Human Recombinant

8009-10
EUR 316

MMP-14, Active, Human Recombinant

8009-50
EUR 805

Active Cathepsin S, human recombinant

7526-10
EUR 338

Active Cathepsin S, human recombinant

7526-50
EUR 1126

Active Cathepsin S, human recombinant

7526-500
EUR 9130

Active Cathepsin B, human recombinant

7580-1000
EUR 9783

Active Cathepsin B, human recombinant

7580-5
EUR 294

Active Cathepsin B, human recombinant

7580-50
EUR 1132

Cathepsin K, Active, human recombinant

7600-5
EUR 392

Cathepsin K, Active, human recombinant

7600-50
EUR 2279

MMP-9, Active, human recombinant

7867-1000
EUR 501

MMP-9, Active, human recombinant

7867-500
EUR 332

Active BCL2L1 Protein, human recombinant

7885-10
EUR 256

Active BCL2L1 Protein, human recombinant

7885-50
EUR 691

Recombinant Human Activin-A Active

7-00004 1ug Ask for price

Recombinant Human Activin-A Active

7-00005 5ug Ask for price

Recombinant Human Activin-A Active

7-00006 100µg Ask for price

Recombinant Human Activin-B Active

7-00010 1ug Ask for price

Recombinant Human Activin-B Active

7-00011 5ug Ask for price

Recombinant Human Activin-B Active

7-00012 100µg Ask for price

5-Lipoxygenase, Active, Human Recombinant

P1373-200
EUR 316

MMP-9, Active, Human Recombinant

P1567-10 10 µg
EUR 348

MMP-9, Active, Human Recombinant

P1567-50 50 µg
EUR 510

Human CellExp? HDAC6, Human Recombinant, Active

P1265-50
EUR 446

Human CellExp? HDAC8, Human Recombinant, Active

P1266-50
EUR 446

Human CellExp? HDAC11, Human Recombinant, Active

P1267-50
EUR 446

PRMT1, mouse recombinant (Active)

4868-10
EUR 153

PRMT1, mouse recombinant (Active)

4868-1000
EUR 3834

PRMT1, mouse recombinant (Active)

4868-50
EUR 392

Active Human Caspases Group I, Active Human

K241-3-25
EUR 370

Active SIRT6 (GST-tagged), human recombinant

7697-100
EUR 479

Active SIRT6 (GST-tagged), human recombinant

7697-1000
EUR 3013

Active SIRT6 (GST-tagged), human recombinant

7697-20
EUR 272

Active SIRT6 (His-tagged), human recombinant

7699-100
EUR 425

Active SIRT6 (His-tagged), human recombinant

7699-1000
EUR 2545

Active SIRT6 (His-tagged), human recombinant

7699-20
EUR 218

Recombinant Human Alpha-1 Antitrypsin, Active

7-06361 20µg Ask for price

Recombinant Human Alpha-1 Antitrypsin, Active

7-06362 100µg Ask for price

Recombinant Human Alpha-1 Antitrypsin, Active

7-06363 1mg Ask for price

Active Sonic Hedgehog (SHH), human recombinant

4010-100
EUR 936

Active Sonic Hedgehog (SHH), human recombinant

4010-1000
EUR 4432

Active Sonic Hedgehog (SHH), human recombinant

4010-25
EUR 327

Recombinant Human Active Focal Adhesion Kinase

7-03955 1µg Ask for price

Recombinant Human Active Focal Adhesion Kinase

7-03956 5µg Ask for price

Recombinant Human Active Focal Adhesion Kinase

7-03957 10µg Ask for price

Activin-A Human Recombinant Protein, Active

PROTP08476-2 Regular: 10ug
EUR 317
Description: Active form Activin-A Human Recombinant produced in e.coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2kDa.;The Active form Activin-A is purified by standard chromatographic techniques.

Activin-B Human Recombinant Protein, Active

PROTP09529 Regular: 10ug
EUR 317
Description: Activin B human Recombinant produced in Nicotiana benthamiana plant is a beta-B single chain (aa 293-406) containing 123 amino acids (molecular formula C615H910N178O177S12). Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.

Human ACTIVE GLP1(ACTIVE GLP1) ELISA Kit

QY-E05350 96T
EUR 361

Active HRV14 3C Protease Recombinant

8012-100
EUR 588

Active HRV14 3C Protease Recombinant

8012-20
EUR 207

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-100
EUR 446

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-1000
EUR 2931

Oxalate Decarboxylase, Active Bacterial Recombinant

7262-20
EUR 245

Active Caspase-1, rat recombinant

7524-100
EUR 555

Active Caspase-1, rat recombinant

7524-25
EUR 338

Cathepsin K, Active, mouse recombinant

7597-5
EUR 359

Cathepsin K, Active, mouse recombinant

7597-50
EUR 2170

Cathepsin K, Active, rat recombinant

7598-5
EUR 359

Cathepsin K, Active, rat recombinant

7598-50
EUR 2170

Aprotinin, Active, Bovine Recombinant (AOF)

7866-1
EUR 115

Aprotinin, Active, Bovine Recombinant (AOF)

7866-5
EUR 158

Homocysteine ?,?-Lyase (rHCYase), Active, Recombinant

P1117-200
EUR 425

PDK, Active, M. rosea Recombinant

P1161-50
EUR 398

D-Serine Dehydratase, Active, Recombinant

P1193-200
EUR 457

D-Serine Dehydratase, Active, Recombinant

P1193-600
EUR 914

Recombinant MeV Active Nucleocapsid Protein

VAng-Lsx0400-inquire inquire Ask for price
Description: Measles Active Nucleocapsid, recombinant protein from E. coli.

Recombinant WNV Active E Antigen

VAng-Lsx0573-inquire inquire Ask for price
Description: WNV Active E Antigen, recombinant protein from E. coli.

Purified Recombinant Human BMP3 protein, Biologically active

BMP35-R-10 10 ug
EUR 408

Purified Recombinant Human BMP4 protein, Biologically active,

BMP45-R-10 10 ug
EUR 895

Purified Recombinant Human BMP4 protein, Biologically active

BMP45-R-5 5 ug
EUR 529

Purified Recombinant Human BMP6 protein, Biologically active

BMP65-R-20 20 ug
EUR 895

Active Transglutaminase 2 (His-tagged), human recombinant

7700-10
EUR 233

Active Transglutaminase 2 (His-tagged), human recombinant

7700-100
EUR 592

Recombinant Human Casein Kinase 2 beta Active

7-03892 3µg Ask for price

Recombinant Human Casein Kinase 2 beta Active

7-03893 10µg Ask for price

Recombinant Human Casein Kinase 2 beta Active

7-03894 20µg Ask for price

Recombinant purified, human NFkB-p65 protein, active

NFKB651-R-5 5 ug
EUR 651

Thioredoxin isoform 1 (Trx), Active, Human Recombinant

P1039-100
EUR 240

Fructose 1,6-bisphophatase 1, Active, Human Recombinant

P1269-10
EUR 153

Fructose 1,6-bisphophatase 1, Active, Human Recombinant

P1269-50
EUR 479

Activin-A Human Recombinant Protein, Plant-Active

PROTP08476-3 Regular: 5ug
EUR 317
Description: Active form Activin-A Human Recombinant produced in Plant is a homodimeric, glycosylated, polypeptide chain containing 2 x 116 amino acids and having a molecular weight of 27.4kDa.;The Active form Activin-A is fused to a 6-His tag at N-terminus and purified by standard chromatographic techniques.

Recombinant (E.Coli) Human ERK1/MAPK3 Active Enzyme

RP-670 3 ug
EUR 590

Recombinant (E.Coli) Human ERK2/MAPK1 Active Enzyme

RP-672 1 ug
EUR 590

Purified, recombinant Human TACE control protein (active)

TACE15-R-10 10 ug
EUR 712

Human Recombinant VEGF121 Protein (Sf9), biologically active

VEGF24-R-10 10 ug
EUR 445

Human Recombinant VEGF121 Protein (Sf9), biologically active

VEGF24-R-100 100 ug
EUR 2425

Human Recombinant VEGF121 Protein (E.Coli), biologically active

VEGF25-R-10 10 ug
EUR 445

Human Recombinant VEGF121 Protein (E.Coli), biologically active

VEGF25-R-100 100 ug
EUR 2425

Human Recombinant VEGF165 Protein (E.Coli), biologically active

VEGF26-R-10 10 ug
EUR 445

Human Recombinant VEGF165 Protein (E.Coli), biologically active

VEGF26-R-100 100 ug
EUR 2242

Human Recombinant VEGF165 Protein (E.Coli), biologically active

VEGF26-R-1000 1000 ug
EUR 9185

Human Recombinant VEGF165 Protein (Sf9), biologically active

VEGF27-R-10 10 ug
EUR 445

Human Recombinant VEGF165 Protein (HEK), biologically active

VEGF27-R-100 100 ug
EUR 2425

Human Recombinant VEGF165 Protein (Sf9), biologically active

VEGF27-R-1000 1000 ug
EUR 9185

Human CellExp? HDAC5, Catalytic Domain, Human Recombinant, Active

P1268-50
EUR 446

PGP Human, Phosphoglycolate Phosphatase Human Recombinant Protein, Active

PROTA6NDG6-1 Regular: 10ug
EUR 317
Description: PGP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-321a.a) and having a molecular mass of 36.5kDa.;PGP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

CSTA Human, Cystatin-A Human Recombinant Protein, Active

PROTP01040 Regular: 20ug
EUR 317
Description: Cystatin A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 118 amino acids (1-98a.a.) and having a molecular mass of 13.1 kDa.;The Cystatin A is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.

Human, Activin-A Human Recombinant Protein, HEK-Active

PROTP08476-1 Regular: 10ug
EUR 317
Description: Activin-A Human Recombinant produced in HEK cells is a non-glycosylated disulfide-linked homodimer, having a total molecular weight of 25kDa.;The Activin-A is purified by proprietary chromatographic techniques.

GDA Human, Guanine Deaminase Human Recombinant Protein, Active

PROTQ9Y2T3-2 Regular: 10ug
EUR 317
Description: GDA Human Recombinant produced in E. coli is a single polypeptide chain containing 477 amino acids (1-454) and having a molecular mass of 53kDa.;GDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

GLUL Human, Glutamine Synthetase Human Recombinant Protein, Active

PROTP15104-1 Regular: 10ug
EUR 317
Description: GLUL Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 373 amino acids (1-373) and having a molecular mass of 42kDa.

GLO1 Human, Glyoxalase-I Human Recombinant Protein, Active

PROTQ04760-1 Regular: 10ug
EUR 317
Description: Glyoxalase-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 20.7 kDa. ;Glyoxalase-1 is purified by proprietary chromatographic techniques.

CST3 Human, Cystatin-C Human Recombinant Protein, Active

PROTQ6FGW9-1 Regular: 10ug
EUR 317
Description: Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa.;The Cystatin-C is purified by proprietary chromatographic techniques.

MGLL Human, Monoglyceride Lipase Human Recombinant Protein, Active

PROTQ99685-1 Regular: 5ug
EUR 317
Description: MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.

Human Tau proteins ELISA kit

CSB-E12011h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Tau proteins in samples from serum, plasma, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Tau proteins ELISA kit

1-CSB-E12011h
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Tau proteins in samples from serum, plasma, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Lys proteins Antibody

abx219526-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Bone Matrix Proteins

20-abx265965
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

Lys proteins Antibody

ABD2705 100 ug
EUR 438

Bone Matrix Proteins

5-00844 4 x 5mg Ask for price

Lys proteins Antibody

DF2705 200ul
EUR 304
Description: Lys proteins antibody detects endogenous levels of total Lys proteins.

Human SOD Protein (Active)

20-abx060221
  • EUR 314.00
  • EUR 732.00
  • EUR 1295.00
  • 100 ug
  • 1 mg
  • 2 mg
  • Shipped within 5-10 working days.

Thrombin, Active, Human Plasma

7593-1000
EUR 1007

Thrombin, Active, Human Plasma

7593-250
EUR 311

Thrombin, Active, Human Plasma

7593-2500 Ask for price

Cathepsin B (active, human)

1021-5
EUR 251

Cathepsin D (active, human)

1022-5
EUR 240

Cathepsin H (active, human)

1023-5
EUR 251

Active Human Calpain 1

1134-100
EUR 539

Active Human Calpain 1

1134-1000 1 mg Ask for price
  • Ca²?-dependent cysteine proteinase with low Ca²?requirement (half maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin. Involves in apoptosis activation.
Description: A cysteine protease involved in apoptosis activation

Active Human HSP70 Protein

SPR-108A 0.05 mg
EUR 300
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: Untagged recombinant human HSP70 Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Active Human HSP70 Protein

SPR-108B 0.1 mg
EUR 388
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: Untagged recombinant human HSP70 Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Active Human HSP70 Protein

SPR-108C 2x0.1 mg
EUR 669
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: Untagged recombinant human HSP70 Protein expressed in E. coli for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Active Human HSP70 Protein

SPR-115A 0.05 mg
EUR 346
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: His tagged recombinant human HSP70 Protein expressed in Baculovirus/Hi5 cells for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Active Human HSP70 Protein

SPR-115B 0.1 mg
EUR 477
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: His tagged recombinant human HSP70 Protein expressed in Baculovirus/Hi5 cells for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Active Human HSP70 Protein

SPR-115C 2x0.1 mg
EUR 813
  • HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplas
  • Show more
Description: His tagged recombinant human HSP70 Protein expressed in Baculovirus/Hi5 cells for WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA. This protein is biologically active (ATPase active).

Pdf molecules derived as a combination of PDF withstand individual spatial features of the whole molecule. The optimization procedure is a method that applies a variable target function conjugate gradient algorithm for the position of all non-hydrogen atoms. This method is automated and illustrated by trypsin modeling of two other serine proteinase.

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