Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.

BLAST program is widely used tool to find protein and DNA databases for sequence similarities. For protein comparisons, various definitions, algorithmic and statistical improvements described here allows the BLAST program execution time will decrease substantially while increasing their sensitivity to weak similarity.

New criteria to trigger the expansion of word hits, combined with new heuristics to generate gapped alignments, resulting in a gapped BLAST program that runs about three times the original speed.

Moreover, the method was introduced to automatically incorporate statistically significant alignments generated by BLAST into position-specific score matrix, and search the database using this matrix.

The resulting Position-Specific Iterated BLAST (PSI-BLAST) program running at approximately the same rate per iteration as gapped BLAST, but in many cases much more sensitive to weak but biologically relevant sequence similarities. PSI-BLAST is used to some new and interesting members of BRCT reveal superfamily.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

A method has been designed for electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method produces quantitative transfer of ribosomal protein gel containing urea. For sodium dodecyl sulfate gels, the original band pattern is obtained without loss of resolution, but the transfer is not quantitative.

This method allows the detection of proteins by autoradiography and simpler than conventional procedures. The immobilized proteins were detected by immunological procedures.

All additional binding capacity on the nitrocellulose was blocked with excess protein; then the specific antibody bound and, finally, a second antibody directed against the first antibody. The second antibody either radioactively labeled or fluorescein or peroxidase conjugate.

Certain proteins are then detected by either autoradiography, under UV light, or with a peroxidase reaction product, respectively. In the latter case, as little as 100 pg of protein clearly detectable. It is anticipated that the procedure will be applicable to the analysis of various proteins with a particular reaction or a ligand.

High-resolution two-dimensional electrophoresis of proteins.

A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Because the resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from biological sources are complex.

Proteins are separated according to the isoelectric point by isoelectric focusing in the first dimension, and according to molecular weight by sodium dodecyl sulfate electrophoresis in the second dimension. Since these two parameters are not related, it is possible to obtain nearly uniform distribution of protein spots on the gel-diminsional.

This technique has been completed in 1100 the different components of Escherichia coli and should be able to complete a maximum of 5000 proteins. A protein that contains as little as one disintegration per minute either 14C or 35S can be detected by autoradiography. A protein which is 10 minus 4 to 10 minus 5% of the total protein can be detected and quantified by autoradiography.

Reproducibility enough separation to allow any place in one of the separation to be matched to a different place in the separation. This technique provides a method to estimate (on the sensitivity described) of the amount of protein made by biological systems.

This system can cope with different proteins in a single charge and can consequently be used in the analysis of in vivo modification results in changes in the cost.

Proteins are responsible for altered by missense mutations can be identified. A detailed description of the methods and characteristics of this system is presented.

Comparative protein modeling by satisfaction of spatial restrictions.

We describe a comparative protein modeling methods designed to find the most likely structure for a particular sequence alignment with related structures.

Three-dimensional (3D) model is obtained with a satisfactory optimal spatial limitation comes from the harmony and expressed as a probability density function (PDF) for a feature retained. For example, the probability for the main-chain conformation residues models can be controlled by the type residue, main-chain conformation of similar residues in proteins related, and local similarity between two sequences.

Some PDF is obtained from the correlation between structural features in 17 families of homologous proteins that are aligned on the basis of their 3D structure. The PDF withstand C-C distance alpha alpha, main-chain N-O distance, the main-chain and side chain dihedral angles.

A smoothing procedure is used in the derivation of this relationship is to minimize problems rarely database. 3D models of protein molecules obtained by optimizing pdf restrictions that violate the model inputs as little as possible.

GFP, Active Human Recombinant Protein
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BTK, Active Human Recombinant Protein
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Axl, Active Human Recombinant Protein
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FAK, Active Human Recombinant Protein
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ITK, Active Human Recombinant Protein
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ITK, Active Human Recombinant Protein
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FAP, Active Human Recombinant Protein
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LCK, Active Human Recombinant Protein
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ITK, Active Human Recombinant Protein
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DDR2 Active Human Recombinant Protein
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TRKA, Active Human Recombinant Protein
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p85a, Active Human Recombinant Protein
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PLK1, Active Human Recombinant Protein
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NEK2, Active Human Recombinant Protein
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aRAF, Active Human Recombinant Protein
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DPP8, Active Human Recombinant Protein
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MKK6 Active Human Recombinant Protein
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WWP1, Active Human Recombinant Protein
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DPP9, Active Human Recombinant Protein
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DPP7, Active Human Recombinant Protein
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Flt3, Active Human Recombinant Protein
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TRKC, Active Human Recombinant Protein
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Aha1, Active Human Recombinant Protein
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Tie2, Active Human Recombinant Protein
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Tyk2 Active Human Recombinant Protein
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PLK2, Active Human Recombinant Protein
GWB-16CD9F	GenWay Biotech	10ug	Ask for price

MDM2 Active Human Recombinant Protein
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Jak1 Active Human Recombinant Protein
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NEK7, Active Human Recombinant Protein
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USP7, Active Human Recombinant Protein
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NEK9, Active Human Recombinant Protein
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TDP2, Active Human Recombinant Protein
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PrCP, Active Human Recombinant Protein
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cMet, Active Human Recombinant Protein
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PKM1, Active Human Recombinant Protein
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cKit, Active Human Recombinant Protein
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PYK2, Active Human Recombinant Protein
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CHK2, Active Human Recombinant Protein
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Rheb, Active Human Recombinant Protein
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Akt3, Active Human Recombinant Protein
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MUSK, Active Human Recombinant Protein
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EGFR, Active Human Recombinant Protein
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PDK1, Active Human Recombinant Protein
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Her4, Active Human Recombinant Protein
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TRKB, Active Human Recombinant Protein
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DPP3, Active Human Recombinant Protein
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Akt1,Active Human Recombinant Protein
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XIAP, Active Human Recombinant Protein
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AHCY, Active Human Recombinant Protein
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ACK1, Active Human Recombinant Protein
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ROS1 Active Human Recombinant Protein
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NEK6, Active Human Recombinant Protein
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DPP4, Active Human Recombinant Protein
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cRAF, Active Human Recombinant Protein
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MTAP, Active Human Recombinant Protein
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Flt1, Active Human Recombinant Protein
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Her2, Active Human Recombinant Protein
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Jak3, Active Human Recombinant Protein
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PLK3, Active Human Recombinant Protein
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ZAP70, Active Human Recombinant Protein
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PDE3B, Active Human Recombinant Protein
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PDE1B, Active Human Recombinant Protein
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DYRK2 Active Human Recombinant Protein
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PDE7A, Active Human Recombinant Protein
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BRSK2, Active Human Recombinant Protein
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ALAS2, Active Human Recombinant Protein
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PDE6C, Active Human Recombinant Protein
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PTPmu, Active Human Recombinant Protein
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MEKK2, Active Human Recombinant Protein
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FGFR4, Active Human Recombinant Protein
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RPTPg, Active Human Recombinant Protein
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RNF20 Active Human Recombinant Protein
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UBE3A Active Human Recombinant Protein
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UCHL3 Active Human Recombinant Protein
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PDE1C, Active Human Recombinant Protein
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PDE3A, Active Human Recombinant Protein
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VPS34 Active Human Recombinant Protein
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EphB2, Active Human Recombinant Protein
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STK33 Active Human Recombinant Protein
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EphB4, Active Human Recombinant Protein
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Hsp40, Active Human Recombinant Protein
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NEDD4, Active Human Recombinant Protein
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USP14 Active Human Recombinant Protein
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IRAK4, Active Human Recombinant Protein
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PDE1A, Active Human Recombinant Protein
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USP10 Active Human Recombinant Protein
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FGFR2, Active Human Recombinant Protein
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EphA2, Active Human Recombinant Protein
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DAPK1 Active Human Recombinant Protein
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PDE7B, Active Human Recombinant Protein
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PDE4B2, Active Human Recombinant Protein
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PDE4D3, Active Human Recombinant Protein
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VEGFR3, Active Human Recombinant Protein
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p70S6K, Active Human Recombinant Protein
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SMURF2, Active Human Recombinant Protein
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PDE8A1, Active Human Recombinant Protein
GWB-60E572	GenWay Biotech	10ug	Ask for price

PDE4D2, Active Human Recombinant Protein
GWB-63994F	GenWay Biotech	0.005 mg	Ask for price

ALOX15 Active Human Recombinant Protein
GWB-6672E3	GenWay Biotech	20ug	Ask for price

PDGFRa Active Human Recombinant Protein
GWB-24DADD	GenWay Biotech	0.01 mg	Ask for price

PDE9A2, Active Human Recombinant Protein
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OTUD6B Active Human Recombinant Protein
GWB-PS2F91	GenWay Biotech	50ug	Ask for price

PDE4D2, Active Human Recombinant Protein
GWB-PS819C	GenWay Biotech	5ug	Ask for price

Hsp90a, Active Human Recombinant Protein
GWB-816F04	GenWay Biotech	200ug	Ask for price

PDE4B1, Active Human Recombinant Protein
GWB-87851A	GenWay Biotech	10ug	Ask for price

PDGFRb, Active Human Recombinant Protein
GWB-8C1FA1	GenWay Biotech	10ug	Ask for price

PDE5A1, Active Human Recombinant Protein
GWB-9C8952	GenWay Biotech	10ug	Ask for price

PDE4D7, Active Human Recombinant Protein
GWB-6754B8	GenWay Biotech	5ug	Ask for price

RbAp48, Active Human Recombinant Protein
GWB-74261D	GenWay Biotech	50ug	Ask for price

SMURF1, Active Human Recombinant Protein
GWB-FF1056	GenWay Biotech	20ug	Ask for price

CoREST, Active Human Recombinant Protein
GWB-EBBBD1	GenWay Biotech	100ug	Ask for price

PDE4C1, Active Human Recombinant Protein
GWB-C9515C	GenWay Biotech	5ug	Ask for price

FKBP12, Active Human Recombinant Protein
GWB-D2E2E9	GenWay Biotech	200ug	Ask for price

Hsp90b, Active Human Recombinant Protein
GWB-C657EE	GenWay Biotech	200ug	Ask for price

PDE10A1 Active Human Recombinant Protein
GWB-2AAF78	GenWay Biotech	10ug	Ask for price

PDE11A4, Active Human Recombinant Protein
GWB-11830F	GenWay Biotech	10ug	Ask for price

PDE4A10 Active Human Recombinant Protein
GWB-PS90FE	GenWay Biotech	10ug	Ask for price

PDE4A4B Active Human Recombinant Protein
GWB-PSF5A3	GenWay Biotech	10ug	Ask for price

PTPbeta, Active Human Recombinant Protein
GWB-PSFF36	GenWay Biotech	20ug	Ask for price

PDE10A2 Active Human Recombinant Protein
GWB-A6F078	GenWay Biotech	10ug	Ask for price

PDE4A1A, Active Human Recombinant Protein
GWB-4DB22F	GenWay Biotech	10ug	Ask for price

PKG1beta, Active Human Recombinant Protein
GWB-64BEAA	GenWay Biotech	10ug	Ask for price

GSK3beta, Active Human Recombinant Protein
GWB-683604	GenWay Biotech	10ug	Ask for price

PKG1alpha, Active Human Recombinant Protein
GWB-23A92A	GenWay Biotech	10ug	Ask for price

GSK3alpha, Active Human Recombinant Protein
GWB-FCAF97	GenWay Biotech	10ug	Ask for price

FYN A, Active Human Recombinant Protein
GWB-PS6991	GenWay Biotech	10ug	Ask for price

Bcl-2, Active Human Recombinant Protein
GWB-532360	GenWay Biotech	100ug	Ask for price

ACC-1, Active Human Recombinant Protein
GWB-2009DC	GenWay Biotech	10ug	Ask for price

MEG-2, Active Human Recombinant Protein
GWB-PS0DE2	GenWay Biotech	20ug	Ask for price

ACC-1, Active Human Recombinant Protein
GWB-PS2090	GenWay Biotech	10ug	Ask for price

MEG-1, Active Human Recombinant Protein
GWB-PSB90D	GenWay Biotech	20ug	Ask for price

ACC-2, Active Human Recombinant Protein
GWB-79EAC4	GenWay Biotech	10ug	Ask for price

HDAC-4, Active Human Recombinant Protein
GWB-3CD9B3	GenWay Biotech	10ug	Ask for price

HDAC-1, Active Human Recombinant Protein
GWB-222284	GenWay Biotech	50ug	Ask for price

MNK1 (5385D), Active Human Recombinant Protein
GWB-18F7F0	GenWay Biotech	10ug	Ask for price

USP2 (Sf9) Active Human Recombinant Protein
GWB-1D6F15	GenWay Biotech	50ug	Ask for price

BRAF/p50, Active Human Recombinant Protein
GWB-932C16	GenWay Biotech	10ug	Ask for price

Cot/Tpl2, Active Human Recombinant Protein
GWB-AF0BA2	GenWay Biotech	10ug	Ask for price

IGF-1R, Active Human Recombinant Protein
GWB-AF1F98	GenWay Biotech	10ug	Ask for price

HDAC-7, Active Human Recombinant Protein
GWB-675906	GenWay Biotech	10ug	Ask for price

Jak2 Jh1, Active Human Recombinant Protein
GWB-FDAB1C	GenWay Biotech	10ug	Ask for price

Bcl-xL, Active Human Recombinant Protein
GWB-B2AE2B	GenWay Biotech	100ug	Ask for price

HDAC-9, Active Human Recombinant Protein
GWB-EAC877	GenWay Biotech	10ug	Ask for price

HDAC-5, Active Human Recombinant Protein
GWB-C87EF0	GenWay Biotech	10ug	Ask for price

HDAC-8, Active Human Recombinant Protein
GWB-C3AA2A	GenWay Biotech	50ug	Ask for price

Pdf molecules derived as a combination of PDF withstand individual spatial features of the whole molecule. The optimization procedure is a method that applies a variable target function conjugate gradient algorithm for the position of all non-hydrogen atoms. This method is automated and illustrated by trypsin modeling of two other serine proteinase.