Vedolizumab immunogenicity was assessed using an enzyme-linked immunosorbent assay (ELISA) with ~ 0.5 ug / mL drug disorders, which may underestimate the on-drug immunogenicity. We aimed to compare the results between ELISA and immunogenicity of a new drug-tolerant electrochemiluminescence (ECL) assay (and two versions of neutralizing tests, drug-sensitive to the drug-tolerant). ECL assay drug tolerance […]Continue Reading
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
BLAST program is widely used tool to find protein and DNA databases for sequence similarities. For protein comparisons, various definitions, algorithmic and statistical improvements described here allows the BLAST program execution time will decrease substantially while increasing their sensitivity to weak similarity. New criteria to trigger the expansion of word hits, combined with new heuristics […]Continue Reading
Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.
Multiple sequence alignments are fundamental to many methods of sequence analysis. Most alignments computed using a heuristic progressive alignment. This method began to be a bottleneck in some pipeline analysis when dealing with thousands of sequence data set size. Some methods allow the calculation of a larger set of data while sacrificing quality, and others […]Continue Reading
Targeting the RNA-Binding Protein HuR Alleviates Neuroinflammation in Experimental Autoimmune Encephalomyelitis: Potential Therapy for Multiple Sclerosis
Multiple sclerosis (MS) is an inflammatory disease and chronic autoimmune neurodegenerative central nervous system characterized by demyelination, axonal loss and motor dysfunction. activated microglia associated with the destruction of myelin in the CNS. activated microglia produce proinflammatory cytokines and factors, supports nerve inflammation, damage to myelin and neuronal loss, and suspected to be involved in […]Continue Reading
Population-Based Prevalence of Chlamydia trachomatis Infection and Antibodies in four Districts with Varying Levels of Trachoma Endemicity in Amhara, Ethiopia
Trachoma Control Program in Amhara Region, Ethiopia, improved surgery, antibiotics, facial cleanliness, and environmental improvement (SAFE) strategy in all districts starting in 2007. Despite these efforts, many districts still requires an additional year of AMAN. In 2017, four districts were selected for assessment of antibody response to an antigen of Chlamydia trachomatis and C. trachomatis […]Continue Reading